rabbit polyclonal antibody for fshr  (Proteintech)

Journal: Biomolecules

Article Title: A Novel Culture System for Inhibiting In Vitro Differentiation of Ovine Granulosa Cells

doi: 10.3390/biom15091280

Figure Lengend Snippet: Localization of FSHR and STAR in sheep granulosa cells during primary culture. Intense immunostaining of FSHR (red) and STAR (red) proteins in sheep fresh GCs ( a , b ), and in primary GCs cultured in serum-containing media for 24 h ( c , d ) and 48 h ( e , f ). Cell nuclei were counterstained with DAPI (blue). The localization of FSHR and STAR in GCs is shown in the enlarged images marked by yellow boxs. Fluorescence intensity was quantified using ImageJ 1.54g ( g ) ( n = 3). Bar = 10 μm. Values are expressed as mean ± SEM, *** p < 0.001, “ns” is the abbreviation of “no significance,” indicating no statistical significance in the difference between the two groups ( p > 0.05).

Article Snippet: Subsequently, the cells were incubated overnight at 4 °C with the primary antibodies rabbit anti-FSHR (1:100, 22665-1-AP, Proteintech, Wuhan, China) and rabbit anti-STAR (1:100, bs-20388R, Bioss, Beijing, China).

Techniques: Immunostaining, Cell Culture, Fluorescence

Journal: Biomolecules

Article Title: A Novel Culture System for Inhibiting In Vitro Differentiation of Ovine Granulosa Cells

doi: 10.3390/biom15091280

Figure Lengend Snippet: Detection of luteinization-related indicators in granulosa cells during primary culture. The protein levels of FSHR ( a ) and STAR ( b ) in primary GCs cultured in serum-containing media for 48 h were analyzed by immunoblotting and quantified. α-tubulin was used as an internal control. P4 ( c ) accumulation in the serum-containing media of primary GCs cultured in vitro for the same duration was measured. Values are expressed as mean ± SEM, ** p < 0.01, *** p < 0.001, “ns” is the abbreviation of “no significance,” indicating no statistical significance in the difference between the two groups ( p > 0.05).

Article Snippet: Subsequently, the cells were incubated overnight at 4 °C with the primary antibodies rabbit anti-FSHR (1:100, 22665-1-AP, Proteintech, Wuhan, China) and rabbit anti-STAR (1:100, bs-20388R, Bioss, Beijing, China).

Techniques: Cell Culture, Western Blot, Control, In Vitro

Journal: Biomolecules

Article Title: A Novel Culture System for Inhibiting In Vitro Differentiation of Ovine Granulosa Cells

doi: 10.3390/biom15091280

Figure Lengend Snippet: A preliminary study on the causes of luteinization and the role of BAY-899 in primary cultured granulosa cells. The LH ( a ) levels in serum-free and serum-containing media were measured by ELISA. The relative mRNA levels of LHR ( b ) in serum-containing media were measured at 12, 24, and 48 h. Primary sheep GCs were cultured in serum-containing media with or without BAY-899 (0–180 nmol/L) for 48 h. The cells were collected to assess FSHR ( c ) and STAR ( d ) protein expression by Western blotting. The P4 concentration ( e ) in the culture supernatants was determined by ELISA after treatment with or without 180 nmol/L BAY-899 for 12, 24, and 48 h. Values are presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Subsequently, the cells were incubated overnight at 4 °C with the primary antibodies rabbit anti-FSHR (1:100, 22665-1-AP, Proteintech, Wuhan, China) and rabbit anti-STAR (1:100, bs-20388R, Bioss, Beijing, China).

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Concentration Assay

Journal: Biomolecules

Article Title: A Novel Culture System for Inhibiting In Vitro Differentiation of Ovine Granulosa Cells

doi: 10.3390/biom15091280

Figure Lengend Snippet: Effect of BAY-899 on the luteinization process of second-generation granulosa cells (GCs). Second-generation sheep GCs were incubated in serum-containing media with (BAY-899+) or without BAY-899 (BAY-899−) (180 nmol/L) for 48 h. Immunostaining of FSHR protein (red, ( a , c )) and STAR (red, ( b , d )) in second-generation GCs cultured in serum-containing media with (BAY-899+) or without BAY-899 (BAY-899−). Cell nuclei were counterstained with DAPI (blue). FSHR and STAR in second-generation GCs are highlighted by the yellow squares in enlarged images. Fluorescence intensity was measured using Image J software ( e ) ( n = 3). Bar = 10 μm. Values are expressed as mean ± SEM, * p < 0.05, ** p < 0.01. The concentration of P4 ( f ) in the culture supernatants was measured by ELISA after second-generation GCs were treated with (BAY-899+) or without 180 nmol/L BAY-899 (BAY-899−) for 48 h. Values are mean ± SEM, ** p < 0.01.

Article Snippet: Subsequently, the cells were incubated overnight at 4 °C with the primary antibodies rabbit anti-FSHR (1:100, 22665-1-AP, Proteintech, Wuhan, China) and rabbit anti-STAR (1:100, bs-20388R, Bioss, Beijing, China).

Techniques: Incubation, Immunostaining, Cell Culture, Fluorescence, Software, Concentration Assay, Enzyme-linked Immunosorbent Assay